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. 2016 Jun 21;7:11932. doi: 10.1038/ncomms11932

Figure 4. Suppression of STING palmitoylation inhibits the type I interferon response elicited by the mutant STING variants associated with SAVI.

Figure 4

(a) HEK293T cells were transfected as indicated, fixed, permeabilized and stained with anti-STING antibody. (b) Co-immunostaining of cells in a with anti-TGN46 (a TGN protein). Nuclei were stained with DAPI (blue). Scale bars, 10 μm. (c–e) HEK293T cells were transfected as indicated, together with an ISRE (also known as PRDIII or IRF-E)-luciferase reporter (c), IFNβ-luciferase reporter (d) and NF-κB-luciferase reporter (e) for 24 h. Luciferase activity was then measured. (f) Cells were transfected as indicated. Cell lysates were prepared 24 h after transfection, and analysed by western blot. (g) HEK293T cells that stably express SAVI–STING (V147L or N154S) were transfected with an ISRE-luciferase reporter for 24 h. Luciferase activity was then measured. 2-BP (50 μM) was added 6 h after the transfection. (h) Cells that stably express SAVI–STINGs were treated with 50 μM 2-BP for 24 h. Cell lysates were then prepared, and analysed by western blot. Data in c,d,e and g are mean±s.e.m. from three independent experiments. ***P<0.001 (one-way analysis of variance).