Characterization of exosomes derived from human umbilical cord mesenchymal stem cells (hUCMSCs).
hUCMSC-exosomes were isolated and purified from hUCMSC supernatant using a 101Bio Exosome Kit.
(A). Identification of the main morphological characteristics of hUCMSC-exosomes by transmission electron microscopy images, Scale bar = 100 nm/200 nm.
(B). Dynamic light scattering (DLS) number distribution measurement of hUCMSC-exosomes have a single peak (~ 60 nm) diameter.
(C). RNA expression profiles of hUCMSC-exosomes by Genechip analysis.
(D). The markers of exosomes are detected using western blot assay.