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. 2016 Jun 2;173(14):2250–2262. doi: 10.1111/bph.13506

Figure 3.

Figure 3

Prenatal dexamethasone (Dex) treatment suppresses osteoclast differentiation in fetal long bone. (a) TRAP staining (purple) of fetal femur. (b) The number of TRAP+ cells along the chondro‐osseous junction and marrow cavity was quantified microscopically. *P < 0.05 versus control; Student's t‐test; n = 6 per group. (c) qRT‐PCR measurement of mRNA expression of Calcr, TRAP and Ctsk in fetal bone from control and fetus treated with prenatal dexamethasone exposure. *P < 0.05 versus control; Mann–Whitney U‐test; n = 12 per group. (d) qRT‐PCR measurement of mRNA expression of RANKL and OPG in fetal bone from control and fetus treated with prenatal dexamethasone exposure. *P < 0.05 versus control; Mann–Whitney U‐test; , n = 22 per group.