Fig. 2.

Metabolic phenotypes of Skn-1 KO mice fed a normal chow diet.

(a) Changes in body weight under normal chow diet conditions. When fed a normal chow diet, Skn-1 KO mice had lower body weight than their WT littermates for 13 weeks after weaning (n > 75). Also see Fig. S2A.

(b) Skn-1 KO mice had a lower body fat percentage than their WT littermates, whereas there was no significant difference in muscle mass percentage between the two genotypes (n = 10–19).

(c and d) Serum levels of total ketone bodies were higher in Skn-1 KO mice than their WT littermates under ad libitum feeding (n = 18–28) (c) and 18-h fasting conditions (n = 13) (d).

(e) Skn-1 KO and WT littermates had similar food intake at 8 weeks of age (n = 5–8).

(f) Skn-1 KO mice exhibited increased energy expenditure compared with their WT littermates (n = 9).

(g) qRT-PCR analysis revealed that the mitochondrial DNA content in skeletal muscle was significantly higher in Skn-1 KO mice than in their WT littermates. The data are presented as the mean ± SEM; **p < 0.01 and *p < 0.05. See also Fig. S2.