Fig. 5.

Reduced insulin secretion and improved glucose tolerance and insulin resistance in Skn-1 KO mice.

(a and b) Blood glucose (a) and plasma insulin (b) after glucose gavage (3.0 mg/g of body weight) when fed a normal chow diet. Blood glucose was comparable between Skn-1 KO and WT littermates for 120 min after administration (n = 21–33) (a). Skn-1 KO mice exhibited a smaller increase in plasma insulin for 60 min after administration compared with their WT littermates (n = 20–21) (b).

(c and d) Blood glucose (c) and plasma insulin (d) after glucose gavage (2.0 mg/g of body weight) of mice fed a HFD. Blood glucose was lower in Skn-1 KO mice than their WT littermates for 120 min after administration (n = 26–30) (c). Plasma insulin was lower in Skn-1 KO mice than their WT littermates before glucose administration (p < 0.05) and tended to be lower at 15 min after administration (p = 0.10) (n = 25–30) (d).

(e) Blood glucose after intraperitoneal administration of insulin (0.75 mU/g of body weight) when fed a HFD. Skn-1 KO mice exhibited improved insulin resistance compared with WT littermates (n = 5). The data are presented as the mean ± SEM; **p < 0.01 and *p < 0.05. See also Fig. S5.