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. 2016 Jun 24;6:28682. doi: 10.1038/srep28682

Figure 1. HAS2 inhibition counteracts REAC-mediated decrease in-SA-Gal staining.

Figure 1

At the indicated passages, confluent (80%) ADhMSCs were exposed for 12 hours in the absence (Control) or presence of REAC (REAC treated), or they were subjected to a 12-hour REAC treatment in the presence of 1 mM 4-MU (HAS2 inhibitor) (REAC treated + 4-MU). Panel A, shows representative (six separate experiments) SA-β-Gal staining (blue color). Panel B, reports the percentage analysis of positively stained cells under each experimental condition (mean ± S.E.; n = 6; P < 0.05).