Figure 1. Myeloid specific conditional RBP-J knockout (RBP-J cKO) did not result in significant abnormality in eye.

(A) Photomicrographs of the RBP-J cKO (cKO) and control (Ctrl) retina showed similar morphology (H&E). Histograms show the thickness of retinal nerve fiber layer (RNFL), inner nuclear layer (INL), and outer nuclear layer (ONL), respectively. Briefly, three sections of retina tissues with optic nerve were randomly selected from per eye for H&E. The thickness was measured and calculated to get the average thickness, and was compared between two groups. (B) The retinal blood vessels visualized by lectin staining and quantification of the branch points per field. Three representative microscopic fields were examined in each retina and branch points per field were counted. The average number of branch points was calculated. Histogram indicates the comparison the average number of branch points in two groups. (C) Representative transmission electron micrograph of the similar subretinal region of RBP-J cKO and control mice. Briefly, three representative fields were randomly selected from each eye for T.E.M. The average thickness was calculated, and was compared between totally 4 mice per group. Data are presented as mean ± SEM (n = 4 eyes from 4 mice per group). NS, no significance.