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. 2016 Jun 24;6:28617. doi: 10.1038/srep28617

Figure 2. Notch activation in the choroidal tissues of mice with induced CNV.

Figure 2

(A) The eyes of wild-type C57/B16 mice were subjected to laser-induced CNV. Eye cups were collected at the indicated time points after laser treatment, and were stained with anti-F4/80 and anti-NICD. Arrows indicated NICD+ macrophages. (B) The two eyes of one mouse were used as one set of RNA retraction. The relative mRNA level of Hes1 and Hey1 was determined by using qRT-PCR, with β-actin as an internal reference control. Each individual experiment was repeated at least three times. Data are presented as mean ± SEM (n = five mice per group). *P < 0.05, **P < 0.01.