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. 2016 Jun 24;6:28617. doi: 10.1038/srep28617

Figure 7. Reduced pro-angiogenic phenotype of RBP-J-deficient macrophages inhibited angiogenesis.

Figure 7

(A) BMDMs were prepared from RBP-J cKO and control mice, and stimulated with PBS (N.C), LPS+IFN-γ (M1) or IL-4 (M2). bEND.3 cells were cultured in the presence of conditional medium (CM) from differentially activated macrophages, and induced to form lumens in vitro. Representative images of bEND.3 cells cultured with CM of N.C, M1 or M2 macrophages were shown. (B) Five representative microscopic fields were selected randomly in each culture condition, and the number of lumen loops was calculated and branch length of cell cords of the enclosed lumens was measured. The average number of lumen loops and the average branch length were calculated and compared. Data are presented as mean ± SEM, n = five individual experiments. *P < 0.05. NS, no significance.