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. 2016 Jun 24;6:28627. doi: 10.1038/srep28627

Figure 4. PDGF up-regulated miR-150 expression and secretion in SMC in IRE1α/XBP1 dependent manner.

Figure 4

(A) PDGF increased miR-150 expression and secretion in SMC in an XBP1 splicing dependent manner. HSMCs were infected with non-target (NTsh), IRE1α (Ish) or XBP1 (Xsh) shRNA lentivirus at 100 IU for 48 hr, and then treated with DMEM supplemented with 0.5% FBS for 24 hr, followed by 20 ng/ml PDGF-BB treatment for 4 hr. The cellular and EV miR-150 levels were assessed by quantitative RT-PCR of three independent experiments. (mean ± SEM, *P < 0.05; ANOVA, Dunnett post-test) (B) The anti-miR-150 abolished PDGF/SMC-mediated EC migration. HSMCs was transfected with anti-miR-150 RNA (anti) and incubated in complete growth medium for 48 hrs, and then treated with 0.5% FBS medium for 24 hr, followed by 20 ng/ml PDGF-BB treatment for 4 hr. The effect of isolated EVs on HUVEC migration was assessed by transwell migration assays. The left panel shows the representative images, while the right panel shows the statistical analysis of migrated cells per 10x view. Control anti-miR RNA (ctl) was included. Scale bar: 25 μm. Data presented are representative images or mean ± SEM of three independent experiments. (*P < 0.05; ANOVA, Dunnett post-test).