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. 2016 Jun 24;6:28627. doi: 10.1038/srep28627

Figure 7. Over-expression of premiR-150 increased VEGF-A expression and VEGF receptor dependent Akt phosphorylation in HUVECs.

Figure 7

(A,B) Over-expression of premiR-150 increased VEGF-A expression and secretion. HUVECs were transfected with premiR-150 or anti-miR-150 in serum free M199 medium for 5 h, then treated with M199 medium supplemented with 5 μg/ml insulin and 5 μg/ml transferrin for 3 h, followed by quantitative RT-PCR analysis of VEGF-A mRNA (A) or ELISA assay to detect VEGF-A in cell culture medium (B) premiR and anti-miR control RNA fragments were included as controls. (C) SU5416 ablated premiR-150-induced Akt and ErK phosphorylation. HUVECs were transfected with premiR-150 or anti-miR-150 in serum free M199 medium for 5 h, then treated with M199 medium supplemented with 5 μg/ml insulin and 5 μg/ml transferrin in the presence of 10 μM SU5416 for 3 h, followed by Western blot analysis of Akt and ErK phosphorylation and VEGF expression. premiR RNA fragments DMSO were included as controls. Data presented are representative images or mean of three independent experiments. (mean ± SEM, *P < 0.05; ANOVA, Dunnett post-test).