Fig. 2.

LEDGF/p75 depletion reduces HIV reactivation from latency. (a) Schematic representation of the single round HIV reporter virus encoding a tCD34 driven by the viral LTR promoter in the nef position. (b) Bar diagram depicting the fold reactivation (as fold increase in % tCD34 positive cells). Nalm control (+/c) and Nalm LEDGF/p75 KO (−/−) cells were infected with a dilution series of single round reporter virus and the % tCD34 positive cells was monitored. 11 days post infection cells were reactivated using different LRAs at concentrations indicated. Data represent averages of 9 replicates from 3 independent experiments and error bars indicate the standard error of the mean (SEM). A statistical analysis was performed using multiple t tests and corrected using Sidak–Bonferroni (* p < 0.05, ** p < 0.005 vs. LEDGF/p75 KO) (SAHA; Suberoylanilide Hydroxamic Acid, PMA; Phorbol 12-Myristate 13-Acetate, Prostratin, DMSO; Dimethyl sulfoxide.). Normalization was based on equal integrated copy (IC) numbers. All viruses are VSV-G pseudotyped. tCD34; truncated Cluster of Differentiation 34.