Table 1. Frequencies of indels induced at on-target and off-target sites by 17nt and matched 20nt sgGFPs.
| Target ID | Target sequence | 293T | iPSC | iMSC | ||||
|---|---|---|---|---|---|---|---|---|
| 20nt | 17nt | 20nt | 17nt | 20nt | 17nt | 20nt | 17nt | |
| sgGFP42-On | GCCGTCCAGCTCGACCAGGAtGG | GTCCAGCTCGACCAGGAtGG | 98.20% | 98.90% | 75.50% | 71.10% | 80.60% | 60.87% |
| sgGFP42-Off1 | TATGTCCAGCTGGACCAGGAgGG | GTCCAGCTGGACCAGGAgGG | 26.55% | 33.53% | 21.40% | 26.64% | 18.94% | 26.79% |
| sgGFP42-Off2 | GAGCTCCAGCTCAACCAGGAtGG | CTCCAGCTCAACCAGGAtGG | ND | ND | ND | ND | ND | ND |
| sgGFP42-Off4 | GGGCTCCAGCTCAACCAGGAtGG | CTCCAGCTCAACCAGGAtGG | ND | ND | ND | ND | ND | ND |
| sgGFP42-Off5 | GTGGTCCAGCTCGCCCAGGTcGG | GTCCAGCTCGCCCAGGTcGG | ND | ND | ND | ND | ND | ND |
| sgGFP42-Off6 | CCTGGCCAGCTAGACCAGGAtGG | GGCCAGCTAGACCAGGAtGG | ND | ND | ND | ND | ND | ND |
| sgGFP42-Off7 | GCTGGACAGCTCTACCAGGAtGG | GGACAGCTCTACCAGGAtGG | ND | ND | ND | ND | ND | ND |
| sgGFP42-Off9 | GGCGCACAGCTCGACCTGGAgGG | GCACAGCTCGACCTGGAgGG | ND | ND | ND | ND | ND | ND |
| sgGFP42-Off10 | GCCTCCCAGCTCCACCAGGCaGG | TCCCAGCTCCACCAGGCaGG | ND | ND | ND | ND | ND | ND |
| sgGFP42-Off12 | GCCATCCAGGAGGACCAGGAtGG | ATCCAGGAGGACCAGGAtGG | ND | ND | ND | ND | ND | ND |
| sgGFP42-Off13 | GCCCTCCATCCCCACCAGGAgGG | CTCCATCCCCACCAGGAgGG | ND | ND | ND | ND | ND | ND |
| sgGFP42-Off14 | GCCGTCCAGCTCTCCCAGGTgGG | GTCCAGCTCTCCCAGGTgGG | ND | ND | ND | ND | ND | ND |
| sgGFP42-Off15 | GCAGTCCAGCTCTAGGAGGAaGG | GTCCAGCTCTAGGAGGAaGG | ND | ND | ND | ND | ND | ND |
| sgGFP42-Off16 | GCCGTGCAGCTCTAGCAGGGaGG | GTGCAGCTCTAGCAGGGaGG | ND | ND | ND | ND | ND | ND |
| sgGFP101-On | GGCGAGGGCGATGCCACCTAaGG | GAGGGCGATGCCACCTAaGG | 99.50% | 99.43% | 69.23% | 44.40% | 79.63% | 53.83% |
| sgGFP101-Off1 | GAGCAGGGGGATGCCACCTAgGG | CAGGGGGATGCCACCTAgGG | ND | ND | ND | ND | ND | ND |
| sgGFP101-Off2 | GATGAGGGAGAGGCCACCTAgGG | GAGGGAGAGGCCACCTAgGG | ND | ND | ND | ND | ND | ND |
| sgGFP101-Off3 | GGGGAGGGAGATTCCACCTAcGG | GAGGGAGATTCCACCTAcGG | 21.61% | 19.41% | 21.72% | 21.59% | 23.96% | 23.77% |
| sgGFP101-Off5 | GGTGAGGGTGATGCCACCCAgGG | GAGGGTGATGCCACCCAgGG | 20.02% | 11.49% | ND | ND | ND | ND |
| sgGFP101-Off6 | AAAGAGGGCAATTCCACCTAcGG | GAGGGCAATTCCACCTAcGG | ND | ND | ND | ND | ND | ND |
| sgGFP101-Off7 | AGGGAGGGCGGGGCCACCTAtGG | GAGGGCGGGGCCACCTAtGG | 29.91% | 29.27% | 33.19% | 33.15% | 33.61% | 35% |
| sgGFP261-On | GACGTAGCCTTCGGGCATGGcGG | GTAGCCTTCGGGCATGGcGG | 99.73% | 99.80% | 50.47% | 59.80% | 75.77% | 61.07% |
| sgGFP261-Off1 | GCTGGAGCCTTCGGGCATGGcGG | GGAGCCTTCGGGCATGGcGG | ND | 17.77% | ND | ND | ND | ND |
| sgGFP261-Off3 | GGAGAAGCTTTCGGGCATGGgGG | GAAGCTTTCGGGCATGGgGG | ND | ND | ND | ND | ND | ND |
| sgGFP261-Off4 | GGTGTGGCCTTGGGGCATGGgGG | GTGGCCTTGGGGCATGGgGG | ND | ND | ND | ND | ND | ND |
| sgGFP261-Off5 | GGTGTAGGCCTCGGGCATGGcGG | GTAGGCCTCGGGCATGGcGG | ND | ND | ND | ND | ND | ND |
| sgGFP261-Off6 | ACAGAAGCCTTCAGGCATGGaGG | GAAGCCTTCAGGCATGGaGG | ND | ND | ND | ND | ND | ND |
| sgGFP261-Off7 | TTTGTAGTCTTCAGGCATGGgGG | GTAGTCTTCAGGCATGGgGG | 11.92% | 22.15% | ND | ND | ND | ND |
| sgGFP261-Off9 | AACGTAGCCTCAGGGCATGGgGG | GTAGCCTCAGGGCATGGgGG | 28.64% | 17.82% | ND | ND | ND | ND |
| sgGFP261-Off10 | CAGGTAGCCTTGGGCCATGGtGG | GTAGCCTTGGGCCATGGtGG | ND | ND | ND | ND | ND | ND |
| sgGFP261-Off11 | CATGTAGCCTTCAGGCATGTgGG | GTAGCCTTCAGGCATGTgGG | ND | 24.34% | ND | ND | ND | ND |
| sgGFP379-On | GAAGGGCATCGACTTCAAGGaGG | GGGCATCGACTTCAAGGaGG | 99.30% | 98.67% | 78.17% | 44.87% | 75.93% | 42% |
| sgGFP379-Off3 | ACGGGGCATCGATTTCAAGGaGG | GGGCATCGATTTCAAGGaGG | 27.44% | ND | 23.92 | ND | 28.71 | ND |
Nucleotides in bold indicate mismatched target sequences. ND = Not detected.