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Optical density values of the white-tailed deer (WTD) serum samples analyzed with indirect ELISA for Borrelia burgdorferi. (A.) ELISA data from 109 WTD serum samples used as negative controls, collected from 2003 to 2015. (B.) ELISA data from 1384 WTD serum samples collected from 2001 to 2013. The dashed line denotes the cut off value used in this study. The samples above this line were analyzed with standardized western immunoblot assay.
