Fig. 3.

Antitumor activity of TCP-1/TNFα in combination with TCP-1/IFNγ in the orthotopic CRC model. a Survival comparison between nontargeted and targeted TNFα and IFNγ combined treatment groups. TCP-1/TNFα and TCP-1/IFNγ combination group significantly improved survival compared with the nontargeted TNFα and IFNγ group. b Representative picture of the tumors in control and combined groups after treatment for 7 days (n ≥ 4 per group). TCP-1/TNFα and TCP-1/IFNγ combination treatment dramatically inhibited tumor growth. c Tumor weight at sacrifice and d Tumor size along with time in all treatment groups (n ≥ 4 per group). e CD34^bright CD45^dim hematopoietic cells in bone marrow. No myelosuppression was observed for TCP-1/TNFα in combination with TCP-1/IFNγ. f Representative picture of TUNEL staining and quantification. Combination treatment increased TUNEL positive cells inside the tumor. g Representative picture of CD31 immunohistochemistry staining and quantification. TCP-1/TNFα in combination with TCP-1/IFNγ decreased tumor blood vessel. Data were presented as mean ± SEM. **P < 0.01. ***P < 0.001