Table 1.

Biochemical composition of seeds, antioxidant properties, and pathogen resistance of W86 plants across three generations of field cultivation.

	3th generation		5th generation		6th generation
	Control	W86	Control	W86	Control	W86
CHS gene expression	100%	38% ± 2.5**	100%	41% ± 5.2**	100%	36% ± 3.4**
Anthocyanins [μg/gDW]	38.5 ± 0.8	68.5 ± 1.2**	40.3 ± 0.7	71.8 ± 1.6**	37.7 ± 0.6	70.9 ± 1.0*
Flavones [μg/gDW]	0.89 ± 0.06	0.99 ± 0.12	0.82 ± 0.08	0.89 ± 0.09*	1.02 ± 0.06	1.04 ± 0.06
Secoisolariciresinol diglucoside [mg/g DW]	182.3 ± 5.7	184.6 ± 4.3*	168.5 ± 5.1	199.1 ± 5.7*	179.9 ± 3.9	192.7 ± 5.6*
Proanthocyanins [mg/gDW]	0.3 ± 0.02	0.48 ± 0.01**	0.29 ± 0.02	0.53 ± 0.02**	0.31 ± 0.01	0.51 ± 0.02**
Hydrolyzable tannins [mg /gDW]	0.58 ± 0.07	2.7 ± 0.12**	0.59 ± 0.10	2.96 ± 0.18**	0.58 ± 0.21	2.76 ± 0.14**
Linoleic acid (18:2) [μg/gDW]	147 ± 3.34	110 ± 3.94*	136 ± 4.07	103 ± 5.7*	157 ± 5.54	92 ± 2.66**
αLinolenic acid (18:3) [μg/gDW]	4.43 ± 0.64	140 ± 6.54**	5.57 ± 0.7	132 ± 3.54**	6.06 ± 0.47	151 ± 5.11**
IC-50 [mg/ml]	0.27 ± 0.04	0.16 ± 0.04*	0.25 ± 0.05	0.12 ± 0.03*	0.27 ± 0.04	0.14 ± 0.02*
Fusarium infection	45%	23%**	34%	16%**	36%	21%*

Results are mean values ± SD (n = 6). Significance of differences between the means was determined using Student’s t-test (*P < 0.05, **P < 0.01).