Figure 3.

Effects of trolox on the phosphorylation of the cyclin-dependent kinase Cdc2. (A) VA-13 and (B) VL-17A cells were cultured in the presence or absence of 25 mM ethanol containing or lacking 50 µM trolox for three days. Lysates were prepared and immunoblot analysis performed. The results of these studies indicated that the inclusion of the antioxidant trolox in the growth media had no effect on the ethanol metabolism-mediated accumulation of Cdc2 in the inactive, phosphorylated form. (C = control cells, E = cells cultured in the presence of ethanol, T = cells cultured in the presence of trolox, and TE = cells cultured in the presence of trolox and ethanol).