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. 2016 Mar 31;6(2):17. doi: 10.3390/biom6020017

Figure 5.

Figure 5

Effects of NAC on the expression of the cyclin dependent kinase inhibitor p21. (A) VA-13 and (B) VL-17A cells were cultured in the presence or absence of 25 mM ethanol containing or lacking 1 mM NAC for three days. Lysates were prepared and immunoblot analysis performed. The results of these studies indicated that the inclusion of the antioxidant NAC in the growth media had no effect on the ethanol metabolism-mediated increase in the expression of p21. (C = control cells, E = cells cultured in the presence of ethanol, N = cells cultured in the presence of NAC, and NE = cells cultured in the presence of NAC and ethanol).