Table 1.

Overview of relevant Lactobacillus plantarum WCFS1 mutants, the involved gene and their phenotypes, classified according to their gene function. Some mutants with mutations in homologous genes and similar phenotypes are combined

Gene(s)	Locus	Gene function	Affected phenotype	Reference
Substrate utilization & respiration
melA	lp_3485	α‐Galactosidase	Melibiose utilization	Lambert et al. (2007)
lacS2	lp_3468	Sugar permease	ND	Lambert et al. (2007)
cydA	lp_1125	Subunit cytochrome (bd type)	Oxidative respiration	Brooijmans et al. (2009)
narG	lp_1497	Subunit nitrate reductase	Nitrate respiration	Brooijmans et al. (2009)
rpoN	lp_0787	Sigma factor 54	Global expression	Stevens et al. (2010)
manR	lp_0585	Mannose operon regulator	Mannose utilization	Stevens et al. (2010)
manIIC	lp_0576	Mannose transport	Mannose utilization	Stevens et al. (2010)
ccpA	lp_2256	Carbon control protein	Glucose repression	Zotta et al. (2012)
lpdB	lp_0271	Gallate decarboxylase	Tannine utilization	Jiménez et al. (2013)
lpdC	lp_2945	Gallate decarboxylase	Tannine utilization	Jiménez et al. (2013)
Quorum sensing & bacteriocin production
lamA	lp_3580	Response regulator	QS/EPS/biofilm production	Sturme et al. (2005)
lamR	lp_3087	Response regulator	QS/EPS/biofilm production	Fujii et al. (2008)
plnGHSTUVWX	lp_0423‐0430	QS pheromone & transport	QS pheromone production	Meijerink et al. (2010)
plnEFI	lp_0419‐0423	ABC transporter	Plantaricin transport	Meijerink et al. (2010)
plnABCD	lp_0415‐0418	Plantaricin & QS module	Plantaricin A production	Maldonado‐Barragán et al. (2009)
Stress response and intestinal tract survival
bsh1	lp_3538	Choloyl glycin hydrolase	Bile resistance	Lambert et al. (2007)
bsh2‐bsh3‐bsh4	lp_0067‐lp_3362‐lp_2572	Penicillin acylase	Acylase activity	Lambert et al. (2008a)
ctsR	lp_1018	Class III stressor	Stress/control ftsH expression	Fiocco et al. (2009)
ftsH	lp_0547	Chaperone protease	Stress resistance	Fiocco et al. (2009)
hsp 18.55	lp_3352	Heat shock protein	Membrane fluidity	Capozzi et al. (2011)
Cell surface proteins & host interaction
lp_0373	lp_0373	Cell Surface protein	ND	Pretzer et al. (2005)
msa	lp_1229	Mannose‐specific adhesion	Agglutination	Pretzer et al. (2005)
srtA	lp_0514	Sortase	Protein anchoring	Pretzer et al. (2005)
lp_2940	lp_2940	Cell surface protein	Mouse GI tract passage	Bron et al. (2007)
lp_1164	lp_1164	Cellobiose EII transporter	Mouse GI tract passage	Bron et al. (2007)
lp_3055	lp_3055	Copper transporter	Mouse GI tract passage	Bron et al. (2007)
napA3	lp_2827	Na/H antiporter	In vitro GI tract survival	Van Bokhorst‐van de Veen et al. (2012a)
pbp2A	lp_1413	Penicillin‐binding protein	In vitro GI tract survival	Van Bokhorst‐van de Veen et al. (2012a)
lp_1699	lp_1699	AraC regulator	In vitro GI tract survival	Van Bokhorst‐van de Veen et al. (2012a)
Cell shape or surface properties modulation
dltD	lp_2016	D‐alanine transfer	Charged techoic acids	Grangette et al. (2005)
alr	lp_0523	Alanine racemase	Cell envelope integrity	Palumbo et al. (2004)
acm2	lp_2645	N‐acetyl glucosaminidase	Autolysin cell separation	Rolain et al. (2012)
lytA	lp_3421	D,L endopeptidase	Cell shape and integrity	Rolain et al. (2012)
lys2	lp_3093	N‐acetylmuramidase	Cell separation	Rolain et al. (2012)
tagF1‐tagF2	lp_0268‐ lp_0269	Glycerol phosphate transferase	Wall techoic acid modification	Tomita et al. (2013)
cps1A	lp_1177	SPS production	Reduced SPS and rhamnose level	Remus et al. (2012)
cps2A	lp_1197	SPS production	Reduced SPS levels	Remus et al. (2012)
cps3A‐cps4A	lp_1215‐ lp_2108	SPS production	Reduced SPS levels	Remus et al. (2012)
gtfA	lp_1299	Glycosyl transferase	Surface protein glycosylation	Lee et al. (2014)
gtfB	lp_1311	Glycosyl transferase	Surface protein glycosylation	Lee et al. (2014)
oatA	lp_0856	O‐acetyl transferase	Cell septation	Bernard et al. (2011)
oatB	lp_0925	O‐acetyl transferase	Cell septation	Bernard et al. (2011)

GI, Gastrointestinal; EPS, Extracellular polymeric substance; SPS, Surface Poly Saccharide; QS, Quorum Sensing; ND, not detected.