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. 2016 Jun 24;6:28436. doi: 10.1038/srep28436

Figure 2. Aurka controls breast cancer stem cells through regulation of Wnt/β-catenin.

Figure 2

(A) On the left, relative quantification of AurkA and wnt3a in MCF-AurkA+ cells (AurkA). MCF-7 carrying empty vector (empty) were considered as control. On the right, (a) Cytofluorimetric panels show distribution of MCF-AurkA or MCF-empty cells depending on CD44-CD24 staining; (b,c) Distribution of CD44+ or CD24+ subpopulation, respectively, in MCF-empty (top panels) and MCF-AurkA cells (bottom panels). Black arrow in (b) highlights the increase of CD44+ cells in AurkA overexpressing cells (AurkA+). Black bar in (c) highlights the decrease of CD24 expression cells in AurkA overexpressing. (B) On the left, relative quantification of AurkA and wnt3a after AurkA silencing at low (sh8, shMin) or high (sh5, shMax) efficiency in MDA-MB-231 cells. MDA-MB-231 cells carrying empty vector (empty) were considered as control. (a) On the right, cytofluorimetric panels showing distribution of MDA-shAurkA versus MDA-empty cells depending on ALDH-expression (bottom panels). +DEAB empty or shAurkA were negative controls (top panel). (b) ALDH positive cells were scored as reported in the right graph. (C) MFE (Mammosphere Forming Efficiency) valued in AurkA+ MCF-7 or in shAurkA MDA-MB-231. Control cells are indicated as empty. Representative images for each sample were showed at 200X magnification (Scale bar: 800 μm). Data are representative of biological triplicates.