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. 2016 Apr 27;59(10):5095–5101. doi: 10.1021/acs.jmedchem.5b01997

Figure 4.

Figure 4

Representative ITC trace, measured using 26 and the bromodomain of PB1(5); 30 consecutive injections of PB1(5) into a solution of 26 in 20 mM HEPES, 150 mM NaCl, 0.05 mM TCEP. Raw heats (left) and normalized injection heats with a nonlinear least-squares fit for a single binding site model (right) are shown.