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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Microbiol Spectr. 2016 Jun;4(3):10.1128/microbiolspec.TBS-0022-2015. doi: 10.1128/microbiolspec.TBS-0022-2015

Figure 4.

Figure 4

DNA binding properties of recombinant His6-tagged SASP4. A) Electromobility shift assays (EMSA) showing binding to biotin-labelled C. perfringens DNA by purified rSASP4 from F4969 (a CPE-positive nonfood-borne human GI disease strain that forms sensitive spores and produces an SASP4 variant with a Gly at residue 36), SM101 or 01E809 (two CPE-positive food poisoning isolates that form resistant spores and produce an SASP4 variant with Asp at residue 36). B) EMSA showing binding by purified SM101 rSASP4 or rSASP2 to (left) C. perfringens AT-rich biotin-labelled DNA or (right) biotin-labeled C. perfringens GC-rich DNA. Reproduced with permission from (42, 78).

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