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. Author manuscript; available in PMC: 2016 Jun 24.
Published in final edited form as: Nat Methods. 2014 Sep;11(9):972. doi: 10.1038/nmeth0914-972

Figure 1. CsChrimson characterization in cultured cells.

Figure 1

(a) Schematic of CsChrimson chimera. (b) Action spectra for Chrimson and CsChrimson, as well as the Chrimson spectrum data from the original manuscript (HEK293 cells; measured using equal photon fluxes of ~2.5 × 1021 photons/s/m2). (c) Schematic of trafficking sequences used to generate the CsChrimson Drosophila transgenics. (d–e) Maximum photocurrents in response to red (625-nm) and far-red (735-nm) light as measured in cultured neurons. (f–g) Turn-on (f) and recovery kinetics (g) in response to 735-nm light. CsChrimson kinetic data were pooled from all trafficking versions. All constructs in this panel were expressed under CaMKII promoter and selected based solely on the presence of co-transfected cytosolic tdTomato expression. Illumination conditions are as labeled in each panel. Box plot whiskers represent minimum and maximum data points. Box limits represent 25th percentile, median, and 75th percentile. n values: Chrimson-GFP (n = 9 cells in d, n = 12 cells in e), CsChrimson-GFP (n = 7 cells in d, n = 8 cells in e), CsChrimson-KGC-GFP (n = 7 cells in d, e), CsChrimson-GFP-ER2 (n = 4 cells in d, n = 3 cells in e), and CsChrimson-KGC-GFP-ER2 (n = 10 cells in d, n = 11 cells in e). Plotted data are mean ± s.e.m. in b, f, and g. ANOVA with Dunnett’s post hoc test with Chrimson-GFP as reference in d, e.