Table 1.
Nanostring nCounter detection of nine DTC-associated transcripts in three different breast tumor cell lines spiked into healthy donor bone marrow at defined cell number dilutions. Numbers indicate total hybridization counts for each transcript in each sample, after subtracting transcript counts from those obtained from healthy bone marrow controls. Transcripts demonstrating detectable expression proportional with dilution of each cell line are indicated in bold. Low hybridization counts that are constant despite changing tumor cell dilutions, even after background subtraction, are attributed to signal from non-malignant bone marrow cellular constituents.
| Cell Line | SKBR3 | MDA-MB231 | ZR75 | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Dilution | 1×10-5 | 2×10-4 | 5×10-3 | 1×10-5 | 2×10-4 | 5×10-3 | 1×10-3 | 1×10-5 | 2×10-4 | 5×10-3 | 1×10-3 |
| KRT17 | - | - | - | - | - | - | - | - | - | - | - |
| KRT19 | 51 | 424 | 1,408 | - | 42 | 202 | 1,588 | 90 | 400 | 1,252 | 5,580 |
| PITX2 | - | - | - | - | - | - | - | - | - | - | - |
| S100A3 | 13 | 5 | 13 | 8 | 14 | 25 | 122 | 9 | 3 | 7 | 5 |
| SCGB2A2 | 0 | 2 | 5 | - | - | - | - | - | 7 | 23 | 112 |
| SNAI2 | 4 | 4 | 7 | 8 | 20 | 49 | 350 | 11 | 3 | 3 | 12 |
| EPCAM | 38 | 20 | 61 | 9 | 19 | 6 | 32 | 3 | 37 | 169 | 761 |
| TWIST1 | 10 | - | 2 | - | 8 | - | 3 | 4 | 7 | 11 | 2 |
| EGFR | 7 | 14 | - | 2 | 26 | 108 | 783 | 14 | 25 | 15 | 49 |