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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Obesity (Silver Spring). 2016 Mar;24(3):597–605. doi: 10.1002/oby.21377

Figure 2. Adipose tissue fibrosis is decreased in DM humans with obesity.

Figure 2

A. Sirius Red staining of human adipose tissue: left: quantified age-adjusted Sirius Red staining, ordinate: Sirius Red staining intensity, arbitrary intensity units (A.U.); *: p<0.005 comparing DM and NDM groups; right: representative Sirius Red-stained VAT from DM, NDM subjects; n=17 DM, 19 NDM subjects for VAT, and n=9 DM, 9 NDM subjects for SAT.

B. Immunohistochemistry for collagens 1, 3, and 6: top: quantified collagen staining intensity in VAT, SAT, ordinate: arbitrary intensity units; *: p<0.050, **: p<0.100 comparing DM and NDM groups; bottom: representative immunohistochemistry images of collagen 1-stained VAT and collagen 3-stained SAT from DM, NDM subjects; n=10 DM, 10 NDM subjects for VAT and SAT.

C. QRTPCR data from VAT, SAT whole tissue RNA; ordinate: fold difference in transcript levels in DM subjects relative to NDM subject group referent=1; *: p<0.050 comparing DM and NDM groups; n=12 DM, 15 NDM subjects for VAT, n=8 DM, 9 NDM subjects for SAT.

D. Linear regression analysis correlating HbA1c (%) with VAT fibrotic transcripts; ordinates: mean fold difference in transcript levels in DM subjects relative to mean transcript level in NDM subject group referent; all correlations with p<0.150 shown.