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. 2016 Jun 1;9(6):659–669. doi: 10.1242/dmm.023382

Fig. 3.

Fig. 3.

Expression levels of transgene in two Drosophila lines. (A) Western blot analysis of total protein extracts from fly heads of GMR-Gal4>UAS_5A and GMR-Gal4>UAS_2B and (B) elav-Gal4>UAS_5A and elav-Gal4>UAS_2B lines. Eye-specific and pan-neuronal expression of the AggIn construct was achieved using the GMR- and elav-Gal4 drivers, respectively. Western blot densitometry was performed using the ImageJ software and the normalized expression of the transgenic protein is reported in the graphs (mean±s.e.m., n=3).