Fig. 4.

Crotonate increases histone crotonylation in mouse kidney. Mice were treated with crotonate at different doses and for different times. Histone crotonylation was measured by western blotting (anti-Kcr). (A) Dose-response curve at 48 h. Mice received vehicle, or 3, 6 or 12 mmol/kg body weight crotonate by intraperitoneal injection. Mean±s.e.m. of four mice per group. *P<0.05 vs control (non-parametric Mann–Whitney U-test). (B) Timecourse curve. Mice were killed 24, 48 or 72 h following intraperitoneal injection injection of 12 mmol/kg body weight crotonate or vehicle (control). Mean±s.e.m. of four mice per group. *P<0.05 vs control (non-parametric Mann–Whitney U-test).