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. 2016 Jun 1;143(11):1884–1892. doi: 10.1242/dev.126847

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Time-lapse imaging in the microdevice reveals dynamic expression patterns. (A) Contrast has been artificially enhanced in this image to illustrate seeding density. (B-G) Images for subsequent days were acquired every 24 h at a constant intensity. (H) Quantification of GFP intensity over time reveals a pattern of expression similar to that found in vivo (mean intensity ±s.d., n=3). (I-K) Inverted images are shown to enhance neurite visibility. Black arrows illustrate regions of omnidirectional neurite growth on day 6 that were redirected towards higher concentrations of PM (red arrows) on day 7.