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. 2016 Jun 15;143(12):2238–2247. doi: 10.1242/dev.129908

Fig. 3.

Fig. 3.

Cell viability after live imaging with CARS. (A,D) Single z-plane DIC using a 1.27 NA water objective and a 1.4 NA oil condenser and (B,E) depth colour-coded images of CARS stacks at wavenumber 2850 cm−1, of an egg before and after in vitro maturation, showing that development can still occur after live imaging with CARS (n=40). (C,F) Histograms of the number of LDs making up clusters in these cells, demonstrating the change in LD distribution over time. 0.1×0.1 µm xy pixel size; 0.5 µm z-step; 0.01 ms pixel dwell time; ∼13 mW (∼9 mW) pump (Stokes) power at the sample. Scale bars: 10 µm. Colour bar shows depth colour-coding from –25 µm-25 µm (0 µm being the equatorial plane). Data from >5 trials, using 1-3 mice each.