Skip to main content
. 2016 Jun 15;143(12):2147–2159. doi: 10.1242/dev.132415

Fig. 4.

Fig. 4.

Overexpression of Hid recapitulates loss of nos function in larval class IV da neurons. (A-C) Confocal z-series projections of representative class IV da neurons in wild-type (WT) (A), hidWT (B), and hidΔNRE (C) larvae. (D) Quantification of the total number of dendritic terminal branches. (E) Class IV da neurons in WT, hidWT, and hidΔNRE larvae imaged at 92 h and 109 h AEL. Arrowheads indicate examples of new branch outgrowth or branch elongation and arrows designate examples of branch retraction or loss. (F) Quantification of the number of new and/or elongating branches, and lost and/or retracting branches between time points. (G) Quantification of the number of dendritic termini in WT, hidWT, and hidΔNRE larvae at 92 h AEL and 109 h AEL. Net change represents the difference in the average number of terminal branches observed between 92 h and 109 h AEL class IV da neurons. ppk-GAL4 was used to express the UAS-hid transgenes and UAS-CD4-GFP. Values in D,F,G are mean±s.e.m., n≥12 for each genotype; *P≤0.05, **P≤0.01, ***P≤0.001. Scale bars: 100 μm.