Fig. 5.

Reducing caspase activity rescues the dendritic defects of nos-deficient class IV da neurons. (A-D) Confocal z-series projections of representative class IV da neurons from wild-type (WT) (A), nos^RNAi (B), nos^RNAi; DIAP^OE (C), and nos^RNAi; p35^OE (D) larvae. (E) Quantification of dendritic terminal branch number. (F-J) Class IV da neurons from WT (F), nos⁻ (nos^RC/nos^RD, G), Dronc^RNAi; nos⁻ (H), Drice^RNAi; nos⁻ (I), and Dcp-1^RNAi; nos⁻ (J) larvae. Knockdown of Dronc in nos^RNAi da neurons resulted in a similar rescue in dendritic branching (data not shown). (K) Quantification of the total number of dendritic terminal branches. GAL4⁴⁷⁷ was used for expression of UAS-RNAi transgenes, overexpression transgenes, and UAS-CD4-GFP in A-D,F-J. (L,M) Class IV da neurons from WT (L) and hid^ΔN14 (M) larvae. ppk-GAL4 was used to express UAS-hid^ΔN14 and UAS-CD4-GFP. (N) Quantification of dendritic terminal branch number. Values in E,K,N are mean±s.e.m., n≥10 for each genotype; ns, not significant; *P≤0.05, ***P≤0.001. Scale bar: 100 μm.