Fig. 7.
Reducing hid function or overexpressing Nos and Pum delays pruning during pupariation. (A-J) Confocal z-series projections of larval (A,C,E,G,I) and pupal (14 h APF; B,D,F,H,J) class IV da neurons. (A,B) WT, (C,D) hidA206/+, (E,F) nosOE, (G,H) pumOE, and (I,J) DIAP1OE. (K) The percentage of dendrite branch length remaining at 14 h APF normalized to the average branch length of neurons in late L3 larvae for each genotype. (L) RT-PCR to detect hid mRNA in wandering L3 larvae (L) and 12 h APF pupae (P). rp49 was used as a loading control. (M) Anti-GFP (green) and anti-Nos (magenta) immunostaining of class IV da neurons in wandering L3 larvae and 12 h APF pupae. Dashed line outlines the soma. All transgenes were expressed using ppk-GAL4; neurons were marked using UAS-CD4-GFP. Values in K are mean±s.e.m., n≥10 for each genotype; ***P≤0.001. Scale bar: 100 μm in A-J; 10 μm in M.