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. 2016 May 17;5(6):678–688. doi: 10.1242/bio.016642

Fig. 2.

Fig. 2.

pkaap depletion resulted in a decreased number of Rab11 vesicles at the plasma membrane and changed the morphology of intracellular Rab11 endosomes. (A-C) Confocal micrographs of cross-section through the fat body cells showing Rab11-GFP vesicles (green in A-C; greyscale in A′-C′) with the plasma membrane outlined by CellMask™ Deep Red (red in A-C). Arrows depict small ≤1 μm2 Rab11 vesicles at the plasma membrane. Arrowheads depict large Rab11-GFP endosomes with area ≥3 μm2. Representative larval fat body cells were from the following genotypes: (A) control, (B) pkaapRNAi chromosome II and (C) pkaapRNAi chromosome III. Scale bar: 5 μm. Histograms showing (D) comparative analysis of the number of small ≤1 μm2 Rab11 vesicles at the plasma membrane, and (E) size of intracellular Rab11 endosomes with area ≥3 μm2. One-way ANOVA and Tukey's multiple comparison test showed significant differences between the means for the genotypes (depicted by different letters on the bars, P<0.0001). Data presented as mean±s.e.m. PM, plasma membrane.