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. 2016 Jun 15;129(12):2438–2447. doi: 10.1242/jcs.181867

Fig. 1.

Fig. 1.

Depletion of kinesin-12 from cultured astrocytes of adult rat spinal cords decreases proliferation but increases migration. (A) The efficiency of kinesin-12 (K-12) siRNA treatment for the indicated number of days in cultured astrocytes was detected by qRT-PCR (a) and western blotting (b). (c) A representative image of western blotting. (B) The efficiency of kinesin-5 (K-5) siRNA treatment for the indicated number of days in cultured astrocytes was detected by qRT-PCR (a) and western blotting (b). (c) A representative image of western blotting. (C) Representative images of EdU staining of astrocyte proliferation after siRNA treatment for 3 days with (a) control (Ctrl) siRNA, (b) kinesin-12 (K-12) siRNA, (c) kinesin-5 (K-5) siRNA and (d) K-12 and K-5 siRNA. (D) Graph showing astrocyte proliferation after siRNA treatment for 3 days. The data are mean±s.d., n=5 (ctrl siRNA, 18.6±1.7%; K-12 siRNA, 13.9±2.9%; K-5 siRNA, 12.4±2.5%; K12 and K-5 siRNAs, 9.8±1.4%). *P<0.05, **P<0.01 (two-tailed t test).