Skip to main content
. 2016 Jun 15;129(12):2307–2315. doi: 10.1242/jcs.185413

Fig. 1.

Fig. 1.

Generation and characterization of an inducible knockdown mouse model for SCRIB. (A) Schematic of the collagen 1 (ColA1) and Rosa26 locus in KH2 ESCs for generation of ishSCRIB mouse. TRE, tetracycline response element. (B) Cell lysates from mammary glands isolated from control and ishSCRIB mice treated with dox for 8 weeks analyzed for expression of SCRIB, EGFP and E-cadherin (E-Cad). (C) Expression of E-cad, KRT14 and SCRIB analyzed by immunohistochemistry in mammary glands isolated from control and ishSCRIB mice treated with dox for 8 weeks. Scale bars: 50 μm. (D) Immunohistochemical staining of GM130 (red) and ZO-1 (green) in mammary glands isolated from control and ishSCRIB mice treated with dox for 8 weeks. Scale bars: 30 μm. (E) Schematic diagram of the method used to quantify changes in Golgi localization (see Materials and Methods for details). The graph on the right represents percentage of mislocalized Golgi (mean±s.e.m.; n=50 in five ducts). P>0.05 by Student's t-test.