(a) Lifespan (days) and (e) volumetric body lengths of controls for each generation after different generations of continuous exposure, average ± s.e. Asterisks indicate significant differences of MGExp-control compared with UnExp-Reference, *p = 0.05, **p = 0.01. (b–d) Lifespan (days) of nematodes exposed to (b) AgNO3, (c) Ag-PVP and (d) Ag2S exposure after different generations of continuous exposure, average ± s.e. (f–h) Volumetric body length of nematodes exposed to (f) AgNO3, (g) Ag-PVP and (h) Ag2S at 48 h after age synchronization after different generations of continuous exposure, average ± s.e. Asterisks indicate generation with a significant (p = 0.05) concentration-dependent effect on lifespan within a generation.