Table 2. mitochondrial nucleotide composition in six tipuloid flies.
| Region | Cylindrotoma | Paradelphomyia | Pedicia | Rhipidia | Symplecta | Tipula | |
|---|---|---|---|---|---|---|---|
| PCGs(J) | A+T% | 72.7 | 75 | 70.2 | 74.9 | 74 | 70.6 |
| G+C% | 27.2 | 25.1 | 29.9 | 25.1 | 26 | 29.4 | |
| AT-skew | -0.09 | -0.11 | -0.06 | -0.13 | -0.13 | -0.11 | |
| GC-skew | -0.14 | -0.08 | -0.15 | -0.08 | -0.06 | -0.18 | |
| 1stcondon position(J) | A+T% | 66.9 | 68.1 | 62 | 67.9 | 64.8 | 63.6 |
| G+C% | 33.5 | 31.6 | 38 | 32.2 | 35.2 | 36.7 | |
| AT-skew | -0.02 | 0.03 | 0.03 | -0.03 | -0.11 | -0.01 | |
| GC-skew | 0.18 | 0.17 | 0.11 | 0.2 | 0.16 | 0.09 | |
| 2ndcondon position(J) | A+T% | 64.3 | 66.3 | 64.3 | 65.9 | 67.8 | 64.5 |
| G+C% | 35.4 | 33.6 | 35.5 | 34.4 | 32.2 | 35.1 | |
| AT-skew | -0.37 | -0.36 | -0.37 | -0.37 | -0.3 | -0.36 | |
| GC-skew | -0.27 | -0.24 | -0.24 | -0.25 | -0.25 | -0.26 | |
| 3rdcondon position(J) | A+T% | 87.5 | 90.2 | 83.8 | 91.8 | 89.7 | 83.7 |
| G+C% | 12.5 | 9.8 | 16.1 | 8.7 | 10.3 | 16.7 | |
| AT-skew | 0.04 | -0.04 | 0.12 | -0.05 | -0.03 | 0 | |
| GC-skew | -0.6 | -0.3 | -0.55 | -0.49 | -0.2 | -0.59 | |
| PCGs(N) | A+T% | 77.5 | 78.3 | 74.2 | 78.7 | 78.1 | 74.5 |
| G+C% | 22.5 | 21.7 | 25.8 | 21.2 | 21.9 | 25.5 | |
| AT-skew | -0.24 | -0.22 | -0.32 | -0.17 | -0.2 | -0.22 | |
| GC-skew | 0.26 | 0.28 | 0.33 | 0.25 | 0.25 | 0.34 | |
| 1stcondon position(N) | A+T% | 74 | 74.1 | 70.6 | 74.3 | 72.8 | 77.5 |
| G+C% | 26.1 | 25.8 | 29.7 | 25.4 | 27.2 | 22.8 | |
| AT-skew | -0.19 | -0.16 | -0.19 | -0.1 | -0.15 | -0.16 | |
| GC-skew | 0.47 | 0.43 | 0.45 | 0.45 | 0.47 | 0.48 | |
| 2ndcondon position(N) | A+T% | 68.7 | 69.4 | 68 | 69.3 | 68.3 | 69.2 |
| G+C% | 31.6 | 30.4 | 32.4 | 31 | 31.7 | 31.2 | |
| AT-skew | -0.43 | -0.44 | -0.44 | -0.41 | -0.43 | -0.3 | |
| GC-skew | 0 | 0.05 | 0.01 | 0.01 | -0.01 | 0.26 | |
| 3rdcondon position(N) | A+T% | 90.3 | 91.6 | 85.1 | 92.8 | 93.4 | 77.1 |
| G+C% | 9.7 | 8.9 | 15.2 | 7.4 | 6.6 | 22.6 | |
| AT-skew | -0.15 | -0.09 | -0.34 | -0.03 | -0.07 | -0.22 | |
| GC-skew | 0.59 | 0.62 | 0.76 | 0.54 | 0.59 | 0.3 | |
| tRNA genes | A+T% | 78.1 | 80.9 | 75 | 80.3 | 75.9 | 75.8 |
| G+C% | 21.9 | 19.1 | 25 | 19.7 | 24.1 | 24.1 | |
| AT-skew | 0 | 0.02 | 0 | -0.01 | 0 | 0.02 | |
| GC-skew | 0.12 | 0.14 | 0.09 | 0.16 | 0.13 | 0.09 | |
| lrRNA | A+T% | 82 | 83 | 80.1 | 82.5 | 81.5 | 80.1 |
| G+C% | 18 | 17 | 19.9 | 17.5 | 18.5 | 19.9 | |
| AT-skew | -0.04 | -0.1 | -0.09 | -0.01 | -0.05 | -0.03 | |
| GC-skew | 0.29 | 0.31 | 0.3 | 0.28 | 0.29 | 0.36 | |
| srRNA | A+T% | 79.3 | 78.9 | 74.6 | 78.7 | 79.2 | 75.7 |
| G+C% | 20.7 | 21.1 | 25.5 | 21.3 | 20.8 | 24.3 | |
| AT-skew | -0.03 | -0.03 | 0 | -0.01 | -0.01 | 0.02 | |
| GC-skew | 0.26 | 0.27 | 0.26 | 0.23 | 0.25 | 0.25 | |
| Whole mitgenome | A+T% | 76.7 | 77.5 | 73.1 | 77.5 | 77.1 | 73.3 |
| G+C% | 23.3 | 22.4 | 26.9 | 22.4 | 22.9 | 26.6 | |
| AT-skew | 0.03 | 0.02 | 0.08 | 0 | 0.01 | 0.02 | |
| GC-skew | -0.18 | -0.16 | -0.21 | -0.16 | -0.14 | -0.23 |
Note: The AT-bias and GC-bias of PCGs were calculated by the formulae: AT-skew = (A-T)/(A+T), GC-skew = (G-C)/(C+G).