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. Author manuscript; available in PMC: 2016 Jun 24.
Published in final edited form as: Expert Rev Vaccines. 2015;14(9):1255–1275.

Table. 2.

A representativelist of rBCG vaccines against bacterial infections.

rBCG
vaccine
candidates		 BCG
strain		 Antigen
gene(s)		 Hosts Key findings Ref.
TB vaccines China Ag85A, Ag85B C57BL/6 mice The rBCG::AB strain provided a
stronger and longer-lasting protection
against a virulent Mtb challenge.		 [146]
Tokyo Ag85A Rhesus monkeys The rBCG-85A prevented an increase
in old tuberculin test after challenge
with Mtb and reaction of Mtb-derived
antigen.		 [147]
Danish ESAT-6 Guinea pigs A DNA-E6 booster induced an
enhanced antigen specific INF-γ
response, but obliterated the protection
imparted by rBCG against
tuberculosis.		 [148]
Danish HspX Mice The rBCG::X produced a more
consistent and enduring protective
effect against infection with Mtb than
the BCG containing the empty vector
pMV261.		 [149]
Danish Ag85B-Rv3425 C57BL/6 mice The rBCG::Ag85B-Rv3425 induced a
predominant Th1 response.		 [150]
Danish Ag85A, Mpt64,
Mtb8.4		 C57BL/6 mice The rBCG-AMM conferred similar, or
better, protective efficacy against Mtb
infection, compared to BCG or
rBCG-A.		 [151]
Danish Ag85B, ESAT-6,
mouse TNF-α		 C57BL/6 mice The rBCG-Ag85B-Esat6-TNF-α
induced a stronger immune response
than rBCG-Ag85B-Esat6 or BCG.		 [154]
N/A Ag85B, ESAT-6 THP-1 cells The rBCG-Ag85B-ESAT-6 showed an
enhanced immunostimulatory activity
in human macrophages.		 [155]
N/A Ag85B
(P1,P2,P3),
Mtb8.4, ESAT-6,
CFP-10, MTP40		 BALB/c mice The rBCG constructs expressing either
T or T and B cell epitopes of Mtb
induced appropriate immunogenicity
against Mtb.		 [152]
China Ag85A, ESAT-6 BALB/c mice The rBCG-AE was not more
protective against Mtb H37Rv
infection than the parent BCG.		 [156]
Pasteur Ag85B Cattle Vaccination with the rBCG-Ag85B
protected cattle better than the wild
type BCG Pasteur.		 [153]
Diphtheria,
Pertussis and
Tetanus
vaccines		 Moreau S1 subunit of
pertussis toxin		 Neonate mice The rBCG induced higher PT-specific
IFN-γ production and an increase in
both IFN-γ (+) and TNF-α (+), CD4(+)
T cells and conferred protection
against a lethal intracerebral challenge
with B. pertussis. 		[169]
Moreau S1 subunit of
pertussis toxin		 Neonate mice An unmarked complemented
rBCG-Delta
lysA-S1PT-lysA(+)(kan(−)) was
constructed, which induced a high
level of cellular immune response and
protection against B. pertussis
challenge.		 [170]
Moreau Tetanus toxin FC,
CRM197 		BALB/c and
NIH mice		 Reciprocal adjuvant effects of
rBCG-Fc and rBCG-CRM197 were
detected.		 [199]
Moreau CRM197 BALB/c mice The rBCG-CRM197 elicited
neutralizing antibody responses.		 [200]
Shiga
vaccines		 Tokyo Shiga toxin 2B
subunit		 BALB/c mice The rBCG-Stx2B vaccine protected
mice against a challenge by
Shiga-toxin-producing E.coli via
intraperitoneal vaccination.		 [12]

BCG: bacillus Calmette-Guerin; PPD: purified protein derivative; NMIBC: non-muscle invasive bladder cancer.