. 2016 May 13;5:141–149. doi: 10.1016/j.bonr.2016.05.002

Table 1.

Cationic mineral content in femoral bone samples from mice in each diet group.

Mineral/diet group	5-months	12-months	18-months
Calcium
AIN76A	199,500	230,300	229,100
AIN76A + AQ	199,400	233,800	234,100
HFWD	193,100	215,900	204,800
HFWD + AQ	209,300	243,800	218,600

Iron
AIN76A	144	266	228
AIN76A + AQ	69	218	167
HFWD	380	331	327
HFWD + AQ	134	201	224^a

Magnesium
AIN76A	3036	3192	3649
AIN76A + AQ	3250	3724	3797
HFWD	2927	3175	3243
HFWD + AQ	3208	3537	3770

Manganese
AIN76A	2.01	1.22	1.16
AIN76A + AQ	1.97	1.27	0.97
HFWD	7.20	4.44	3.56
HFWD + AQ	1.63	1.36	1.07^a

Potassium
AIN76	759	1273	1458
AIN76A + AQ	724	1109	1227
HFWD	758	1172	1306
HFWD + AQ	903	1123	1245

Silicon
AIN76A	4.75	1.20	1.76
AIN76A + AQ	< 0.5	< 0.5	0.81
HFWD	4.34	2.40	1.78
HFWD + AQ	< 0.5	< 0.5	< 0.5^a

Strontium
AIN76A	50	25	25
AIN76A + AQ	210	260	237^c
HFWD	112	66	44
HFWD + AQ	231	244	238^a,b

Zinc
AIN76A	111	134	124
AIN76A + AQ	124	150	138
HFWD	246	232	198
HFWD + AQ	140	164	147^a,b

Femora from all mice in a group were pooled together, digested and analyzed by ICP-OES, as one sample per group (10 mice per group at 5- and 12-months and 15 mice at 18-months). Boron, copper, chromium and selenium were below detectable limit (0.5 μg/g). The data for each mineral from the three time-points grouped together were subjected to two-way factorial ANOVA followed by paired group comparisons. “a” and “b” are placed on the HFWD + AQ group: “a” shows statistically significant difference relative to the HFWD group, and “b” shows statistically significant difference relative to AIN76A. A “c” is placed on the AIN76A + AQ group shows significant difference relative to AIN76A alone (p < 0.05).