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. 2016 Jun 24;10(6):e0004816. doi: 10.1371/journal.pntd.0004816

Fig 2. Detection of genomic RNA of Zika virus in urine and saliva samples by RT-PCR analysis.

Fig 2

(A) Shows the profiles obtained from urine samples. The lane numbers indicate the patient code. The lane 1I is the amplicon obtained from the viral isolate from urine of patient 1 (isolate Rio-U1). (B) RT-PCR analysis from patients 5 to 9 where S indicates saliva RNA samples, U, urine RNA samples, and I viral isolate sample. (C) Amplification of Zika virus genome of isolate Rio-U1 (1I) with ZIKV-specific primers that were also employed in the RT-PCR assay of Chikungunya virus RNA (CHIKV), dengue virus RNA (DENV) and Yellow Fever 17DD RNA (YFV). In all of these analyses, a negative control of amplification were included (C). The size marker migration is indicated on the left of the figures.