Fig 8. Transmission study–cohabitation plus direct transfer of secretions: Serology.

Sera were tested for anti-NiV IgM (A) and IgG (B) binding antibodies on a Luminex platform. The cut-off for a positive result, represented on each of the graphs as a dashed line, was calculated for each analyte based on binding antibody results from pre-challenge serum samples collected from 17 animals in the study, which were run on the same plate with target samples to generate base-line parameters upon which cutoffs were determined. These were defined as the mean median fluorescence intensity for the 17 pre-challenge samples + 3 standard deviations of the mean. Ig, immunoglobulin.