Fig 8. Theoretical heterodimer formation obtained from the association constants (SE).

(A) Proportion of heterodimer as a function of protein concentration in the absence of detergent. Blue, range of concentrations used in SE; red, range of concentrations used in SLS (overlap is purple); (○) and (●) are the concentration after gel filtration (1:1 act-A/act-B eluted fraction in Fig 2B) and the concentration used in SV, respectively; (B) proportion of heterodimer in detergent as a function of the protein/detergent molar ratio, calculated as described [40]. Blue has the same meaning as in (A), whereas (○) indicates the lipid/protein ratio used in SV.