Fig 5. Spurious super-mutants due to very large UID families.

One very large UID family on the top row is erroneously counted as twelve additional families. The next nine rows show families with UID sequences that differ at one site from the family in the top row, most likely due to a mistake during PCR amplification. The bottom three rows show families with the same long UID sequence but a different short UID sequence from the family in the top row, most likely due to PCR jumping. All of these families contain the same A>T/T>A super-mutant at the read position 10 bases from the end of the UID (p.10A>T) erroneously increasing its frequency.