Table 4. Estimates of the PCR error rate for the different mutation types.
Mutation type | PCR error rate (per site per cycle) | Mutation frequency / PCR error rate |
---|---|---|
A>C/T>G | 6.9 x 10−8 | 6.4 |
C>G/G>C | 3.0 x 10−7 | 3.7 |
A>T/T>A | 7.5 x 10−7 | 1.9 |
A>G/T>C | 1.5 x 10−6 | 2.2 |
C>T/G>A (non-CpG) | 1.0 x 10−6 | 17.0 |
G>T/C>A | 1.3 x 10−6 | 16.9 |
C>T/G>A (CpG) | 2.6 x 10−6 | 28.5 |