Table 5. Read and UID family statistics for the three experiments.
PTPN11 experiment | MECP2 experiment | FGFR3 experiment | |
---|---|---|---|
Initial molecules | 2.3 x 106 | 3.2 x 107 | 3.2 x 107 |
Pairs aligned reads | 8.3 x 107 | 1.3 x 107 | 4.6 x 107 |
Quality readsa | 6.4 x 107 | 7.6 x 106 | 2.8 x 107 |
UID families | 6.1 x 105 | 7.3 x 105 | 2.5 x 106 |
Utility ratiob | 106 | 10.4 | 11.4 |
Average family size | 105 | 7.1 | 8.1 |
Fraction families with sizes 3–10 | 36% | 85% | 80% |
11–99 | 46% | 15% | 20% |
100 + | 18% | < 0.1% | < 0.1% |
Fraction reads with less than 3 reads with same UID | 1% | 32% | 30% |
a Quality reads: number of paired aligned reads such that all base pairs in the barcode and UID have quality scores greater than 32.
b Utility ratio: Quality reads / UID families