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. 2016 Jun 24;11(6):e0158344. doi: 10.1371/journal.pone.0158344

Fig 1. Transduction with CCoq9WP vector increases the levels of Coq9 mRNA and COQ9 protein in MEFs and mHPCs from Coq9R239X mice.

Fig 1

Coq9 mRNA levels in MEFs (A) and mHPCs (B). COQ9 protein levels in MEFs (C) and mHPCs (D). Q: 1 hit in MEFs, 200 μl, non-concentrated; R: 1 hit in MEFs, 100 μl, 10x-concentrated; S: 1 hit in MEFs, 25 μl, 10x-concentrated; T: 1 hit in MEFs, 5 μl, 10x-concentrated; U: 1 hit in HSCs, 400 μl, 23.5x-concentrated; V: 2 hits in HSCs 400 μl, 23.5x-concentrated; 1: 7–9 days after transduction in HSCs; 2: 12–16 days after transduction in HSCs. Data are expressed as mean ± SD. ***P < 0.001, Coq9R239X and Coq9R239X-CCoq9WP cells versus Coq9+/+ cells; +++P < 0.001, Coq9R239X-CCoq9WP cells versus Coq9R239X cells; (one-way ANOVA with a Tukey's post hoc test; n = 4–6 for each group). #: a non-specific band is detected in Coq9R239X cells with the anti-COQ9 antibody.