TABLE 2.
Summary of the Sequences with Highest Homology for Each PCR Product Obtained from the Amplification of a DNA Sample of Colonic Bacteria, with 5 Pairs of Primers
| PCR products obtained from colonic microbiota DNA | Primer direction | Phylum/class | Genus | Identity % | Accession | E value |
|---|---|---|---|---|---|---|
| Regions V1–V2 | F | Bacilli | Lactobacillus | 75 | HQ717267.1 | 8E-08 |
| R | Phylum: Firmicutes | 96 | LN800877.1 | 6E-35 | ||
| Region V4 | F | Bacilli | Lactobacillus | 90 | KC561107.1 | 2E-78 |
| R | Lactobacillus | 88 | HQ851026.1 | 1E-70 | ||
| Region V7 | F | Phylum proteo bacteria | Desulfobacca acetoxidans | 94 | CP002629.1 | 5E-12 |
| R | Unculture A. proteobacteria | 85 | EF219728.1 | 3E-04 | ||
| Regions V7–V8 | F | Bacilli | Lactobacillus | 85 | GU454851.1 | 1E-66 |
| R | 84 | JX079468.1 | 1E-52 | |||
| Lactobacillus spp | F | Not sequenced | ||||
| R | Bacilli | Lactobacillus | 93 | KM250395.1 | 4E-106 | |
F, forward; R, reverse. Identity %, The extent to which 2 sequences have the same residues at the same positions in an alignment, expressed as a percentage.