TABLE 3.
Data Obtained from the SC for E. coli DH5a
| E. coli DH5a DNA concentration (ng) | CFU/ml | Ct general V1–V2 | Ct general V4 | Ct total bacteria 16S V7 | Ct consensus sequence of 7 strains V7–V8 |
|---|---|---|---|---|---|
| 128.8 | 6.3 × 108 | 10.96 ± 0.48 | 13.9 ± 0.72 | 12.23 ± 0.38 | 13.05 ± 0.34 |
| 12.88 | 1.1 × 107 | 14.19 ± 0.36 | 17.54 ± 0.12 | 15.65 ± 0.05 | 16.21 ± 0.17 |
| 1.28 | 1.5 × 106 | 18.35 ± 0.09 | 21.81 ± 0.57 | 19.27 ± 0.02 | 19.9 ± 0.07 |
| 0.12 | 3.5 × 105 | 23.41 ± 0.11 | 28.155 ± 1.71 | 23.00 ± 0.07 | 24.04 ± 0.33 |
| 0.012 | 5.3 × 104 | 27.60 ± 0.07 | 31.71 ± 0.16 | 26.80 ± 0.06 | 27.68 ± 0.00 |
| 0.0012 | 4 × 103 | 32.15 ± 0.47 | >40 | 30.81 ± 0.035 | 32.27 ± 0.04 |
| qPCR negative control | – | >40 | >40 | 34.15 ± 0.60 | >40 |
The table shows the Ct values for PCR products of 4 pairs of primers and their relation with the bacteria DNA concentration and the number of CFU/ml. Ct is defined as the number of cycles required for the fluorescent signal to cross the threshold.