Fig. 2.

H9 hESC-derived cells desensitise the host to other hESC lines.

Graft survival of (A) CsA immune-suppressed (CsA H9; n = 8 + 3), (B) identical (Des H9; n = 8 + 2, H9 to H9-desensitised) and (C) different (Des RC17; n = 8 + 4, RC17 to H9-desensitised) hESC donor – host combinations. (E) A subset of animals was sacrificed after 6 weeks. The percentage of survival was similar in all groups 18 weeks post-transplantation (E, n = 8 per group). Graft sizes were different between the three groups, with RC17 transplants being significantly larger, whilst the graft areas were similar for H9 grafts in CsA or desensitised hosts. Grafts of H9 hESC origin into naïve animals were readily rejected within 6 weeks post transplantation (D; see also Fig. 3). Scalebar = 100 μM. All data in E are presented as mean ± SEM.