Figure 3. Expression of desmosome proteins in FAPs isolated from the mouse heart.
A, B. Detection of expression of desmoplakin (DSP, 2 different antibodies), junction protein plakoglobin (JUP), plakophilin 2 (PKP2) and desmoglein 2 (DSG2) by immunofluorescence (A) and immunoblotting (B) in FACS isolated cardiac FAPs. C. Absence of expression of the sarcomeric proteins MYH6 and MYBPC3 in isolated FAPs by immunoblotting to exclude potential contamination with CMs. Heart tissue is included as a positive control.